Coding

Part:BBa_K1179055

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)

VN173

This part is submitted under the RFC 65 standard.

The Venus fluorescence protein is an improved YFP construct that was created by modifying GFP. The protein has been split at the 172nd residue. The N-terminal venus can fuse with VC155 to produce yellow fluorescence. We express the split venus constructs with a constitutive CAG promoter. A single split venus construct can not reconstitute to form a functional protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None